Peer-Reviewed Journal Details
Mandatory Fields
Coughlan, H,Reddington, K,Tuite, N,Boo, TW,Cormican, M,Barrett, L,Smith, TJ,Clancy, E,Barry, T
2015
October
Diagnostic Microbiology And Infectious Disease
Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae
Published
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H. influenzae Genome comparison Real-time PCR phoB pstA POLYMERASE-CHAIN-REACTION PCR DETECTION HAEMOLYTICUS DISEASE EPIDEMIOLOGY DIFFERENTIATION CARRIAGE STRAINS GENES ASSAY
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Haemophilus influenzae is recognised as an important human pathogen associated with invasive infections, including bloodstream infection and meningitis. Currently used molecular-based diagnostic assays lack specificity in correctly detecting and identifying H. influenzae. As such, there is a need to develop novel diagnostic assays for the specific identification of H. influenzae. Whole genome comparative analysis was performed to identify putative diagnostic targets, which are unique in nucleotide sequence to H. influenzae. From this analysis, we identified 2 H. influenzae putative diagnostic targets, phoB and pstA, for use in real-time PCR diagnostic assays. Real-time PCR diagnostic assays using these targets were designed and optimised to specifically detect and identify all 55 H. influenzae strains tested. These novel rapid assays can be applied to the specific detection and identification of H. influenzae for use in epidemiological studies and could also enable improved monitoring of invasive disease caused by these bacteria. (C) 2015 Elsevier Inc. All rights reserved.
10.1016/j.diagmicrobio.2015.06.013
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