Conference Contribution Details
Mandatory Fields
Kate Reddington*, Elizabeth Minogue, Nina Tuite, Thomas Barry
International Biodeterioration & Biodegradation Society and Public Health England Meeting: “Water Microbiology – Current and Emerging Issues in Healthcare
Real-time PCR diagnostics assays to quantitatively detect and identify human pathogenic microorganisms from water, water distribution and premise plumbing systems
University of Winchester, UK
Oral Presentation
Optional Fields
<!-- /* Font Definitions */ @font-face {font-family:Batang; mso-font-alt:바탕; mso-font-charset:129; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1342176593 1775729915 48 0 524447 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:Batang; mso-ansi-language:EN-GB; mso-fareast-language:KO;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:11.0pt; mso-ansi-font-size:11.0pt; mso-bidi-font-size:11.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:10.0pt; line-height:115%;} @page WordSection1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --> Water is essential to human health, development and wellbeing and plays a crucial role in the industrial, manufacturing, residential and clinical sectors. The presence of pathogenic microorganisms in building water distribution and premise plumbing systems can pose a significant risk to human health particularly in healthcare facilities. In such settings, microorganisms of interest that are typically found include P. aeruginosa, Legionella species, Non Tuberculosis Mycobacteria, Stenotrophomonas maltophilia and also some protozoa and amoeba. Many of these microorganisms are resistant to disinfectants typically used to treat water and as such they have an ability to form biofilms and persist in water, water distribution and premise plumbing systems.   The current century old gold standard microbiological culture-based methodologies for water testing take several weeks to deliver a result and may not be suitable for the detection of protozoa or amoeba. Previous studies have demonstrated that the microbial load present in water can be significantly underestimated using these culture methods due the presence viable but non-culturable bacteria. There are also a number of biochemical and immunodetection methodologies for water based testing however some of these methodologies are unspecific and many are not quantitative.   Nucleic Acid Diagnostics (NAD) technologies offer the potential to rapidly and quantitatively analyse in real-time the onset and progress of microbial biocontamination associated with water, water distribution and premise plumbing systems. Such NAD technologies could be used in the healthcare, residential and industrial settings to rapidly screen water for microbial contamination. Furthermore, the ability to specifically detect, identify and quantify levels of pathogenic microorganisms in such systems rapidly may aid in determining the potential source of contamination and thereby allows for the required infection control and prevention measures to be taken promptly.   In this study, we have developed novel culture independent methodologies which allows for the rapid (< 4 h), quantitative detection and identification of a number of human pathogens from biocontaminated water, water distribution and premise plumbing systems. We propose that the methodologies developed could be applied to routine testing of water to assure microbiological safety and high water quality standards.
Publication Themes