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Ludden, C,Cormican, M,Vellinga, A,Johnson, JR,Austin, B,Morris, D
2015
April
BMC Infectious Diseases
Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year
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Extended-spectrum beta-lactamase (ESBL) Carbapenemase-producing Enterobacteriaceae (CPE) Vancomycin-resistant enterococci (VRE) Meticillin-resistant S. aureus (MRSA) Long-term care facilities LACTAMASE-PRODUCING ENTEROBACTERIACEAE SPECTRUM BETA-LACTAMASES SEQUENCE TYPE 131 ESCHERICHIA-COLI NURSING-HOMES KLEBSIELLA-PNEUMONIAE ANTIMICROBIAL RESISTANCE MOLECULAR EPIDEMIOLOGY RAPID DETECTION MULTIPLEX PCR
15
Background: This study examined colonisation with and characteristics of antimicrobial-resistant organisms among residents of a long-term care facility (LTCF) over one year, including strain persistence and molecular diversity among isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae.Methods: Sixty-four residents of a LTCF were recruited (51 at baseline, 13 during the year). Data on dependency levels, hospitalisations, and antimicrobial prescribing were collected. Nasal and rectal swabs and catheter urine specimens were examined quarterly, using chromogenic agars, for ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant enterococci (VRE), and meticillin-resistant S. aureus (MRSA). All ESBL-producing E. coli (ESBL-EC) were characterised by pulsed-field gel electrophoresis (PFGE) and PCR to assess for sequence type (ST) ST131, its resistance-associated H30 and H30-Rx subclones, and bla(CTX-M), bla(TEM), bla(SHV), and bla(OXA-1).Results: The overall number of residents colonised, by organism, was as follows: ESBL-EC, 35 (55%); MRSA, 17 (27%); ESBL-producing K. pneumoniae (ESBL-KP), 5 (8%); VRE, 2 (3%) and CPE, 0 (0%). All 98 ESBL-EC isolates were H30-Rx ST131, with bla(CTX-M-group) 1. By PFGE, a group of 91 ESBL-EC (from 33 participants) had >= 85% similar profiles and resembled UK epidemic strain A/ international pulsotype PFGE812. Sequential ESBL-EC from individual residents were closely related. Six ESBL-KP isolates, from five participants, had bla(CTX-M-group) 1 and by PFGE were closely related. Colonisation with ESBL and MRSA was associated with location within the LTCF and previous exposure to antimicrobials.Conclusions: Among LTCF residents, colonisation with ESBL-EC and MRSA was common. All ESBL-EC were H30-Rx ST131, consistent with clonal dissemination.
10.1186/s12879-015-0880-5
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