Peer-Reviewed Journal Details
Mandatory Fields
Dimov, N,Clancy, E,Gaughran, J,Boyle, D,Mc Auley, D,Glynn, MT,Dwyer, RM,Coughlan, H,Barry, T,Barrett, LM,Smith, TJ,Ducree, J
2015
May
Microfluidics And Nanofluidics
Solvent-selective routing for centrifugally automated solid-phase purification of RNA
Published
Optional Fields
Lab-on-a-disc RNA purification Solvent-selective valves Molecular diagnostics WHOLE-BLOOD POTENTIAL BIOMARKER DNA EXTRACTION FLOW-CONTROL LAB CANCER AMPLIFICATION POLYMERASE EXPRESSION MICRORNA
18
859
871
We present a disc-based module for rotationally controlled solid-phase purification of RNA from cell lysate. To this end, multi-stage routing of a sequence of aqueous and organic liquids into designated waste and elution reservoirs is implemented by a network of strategically placed, solvent-selective composite valves . Using a bead-based stationary phase at the entrance of the router, we show that total RNA is purified with high integrity from cultured MCF7 and T47D cell lines, human leucocytes and Haemophilus influenzae cell lysates. Furthermore, we demonstrate the broad applicability of the device through the in vitro amplification of RNA purified on-disc using RT-PCR and NASBA. Our novel router will be at the pivot of a forthcoming, fully integrated and automated sample preparation system for RNA-based analysis .
10.1007/s10404-014-1477-9
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