Peer-Reviewed Journal Details
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Kontturi, LS,Jarvinen, E,Muhonen, V,Collin, EC,Pandit, AS,Kiviranta, I,Yliperttula, M,Urtti, A
2014
April
Drug Delivery And Translational Research
An injectable, in situ forming type II collagen/hyaluronic acid hydrogel vehicle for chondrocyte delivery in cartilage tissue engineering
Published
WOS: 30 ()
Optional Fields
Cartilage tissue engineering Chondrocyte Injectable hydrogel Type II collagen Hyaluronic acid Transforming growth factor beta(1) ARTICULAR-CARTILAGE COLLAGEN TYPE HYALURONIC-ACID CANINE CHONDROCYTES CROSS-LINKING STEM-CELLS TGF-BETA SCAFFOLDS BEHAVIOR REPAIR
4
149
158
In this study, chondrocytes were encapsulated into an injectable, in situ forming type II collagen/hyaluronic acid (HA) hydrogel cross-linked with poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4SPEG) and supplemented with the transforming growth factor beta(1) (TGF beta(1)). The chondrocyte-hydrogel constructs were cultured in vitro for 7 days and studied for cell viability and proliferation, morphology, glycosaminoglycan production, and gene expression. Type II collagen/HA/4SPEG formed a strong and stable hydrogel, and the chondrocytes remained viable during the encapsulation process and for the 7-day culture period. In addition, the encapsulated cells showed spherical morphology characteristic for chondrocytic phenotype. The cells were able to produce glycosaminoglycans into their extracellular matrix, and the gene expression of type II collagen and aggrecan, genes specific for differentiated chondrocytes, increased over time. The results indicate that the studied composite hydrogel with incorporated chondrogenic growth factor TGF beta(1) is able to maintain chondrocyte viability and characteristics, and thus, it can be regarded as potential injectable cell delivery vehicle for cartilage tissue engineering.
10.1007/s13346-013-0188-1
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