Peer-Reviewed Journal Details
Mandatory Fields
Ramer, MS,Bishop, T,Dockery, P,Mobarak, MS,O'Leary, D,Fraher, JP,Priestley, JV,McMahon, SB
2002
February
Mol Cell Neurosci
Neurotrophin-3-mediated regeneration and recovery of proprioception following dorsal rhizotomy
Published
Optional Fields
NERVE GROWTH-FACTOR PRIMARY SENSORY NEURONS RAT SPINAL-CORD AXONAL REGENERATION ADULT-RAT CUTANEOUS MECHANORECEPTORS TRK RECEPTORS ROOT AXONS EXPRESSION OUTGROWTH
19
239
249
Injured dorsal root axons fail to regenerate into the adult spinal cord, leading to permanent sensory loss. We investigated the ability of intrathecal neurotrophin-3 (NT3) to promote axonal regeneration across the dorsal root entry zone (DREZ) and functional recovery in adult rats. Quantitative electron microscopy showed robust penetration of CNS tissue by regenerating sensory axons treated with NT3 at 1 and 2 weeks postrhizotomy. Light and electron microscopical anterograde tracing experiments showed that these axons reentered appropriate and ectopic laminae of the dorsal horn, where they formed vesicle-filled synaptic buttons. Cord dorsum potential recordings confirmed that these were functional. In behavioral studies, NT3-treated (but not untreated or vehicle-treated) rats regained proprioception. Recovery depended on NT3-mediated sensory regeneration: preventing regeneration by root excision prevented recovery. NT3 treatment allows sensory axons to overcome inhibition present at the DREZ and may thus serve to promote functional recovery following dorsal root avulsions in humans.
10.1006/mcne.2001.1067
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