Peer-Reviewed Journal Details
Mandatory Fields
Balfe, P,McCann, A,McGoldrick, A,McAllister, K,Kennedy, M,Dervan, P,Kerin, MJ
2004
June
Ejso
Estrogen receptor alpha and beta profiling in human breast cancer
Published
Optional Fields
ER-alpha ER-beta breast cancer Nottingham prognostic index ER-BETA MESSENGER-RNA TRANSCRIPTIONAL ACTIVATION WILD-TYPE EXPRESSION PROLIFERATION LOCALIZATION CLONING PROTEIN VARIANT
30
469
474
Background. The identification of a second estrogen receptor (ER-beta) has significant implications for therapeutic strategy in breast cancer management arising from the potential. agonist effect of Tamoxifen at estrogen receptor sites and as such, antiestrogen therapy may be inappropriate in patients with a dominance of ER-beta.Methods. To determine the proportion of breast cancer patients who may be so at risk, we developed a novel multiptexed RT-PCR technique to establish the relative ER-alpha. and ER-beta levels in 53 primary breast cancers, 11 normal breast tissues and six cell. lines. We further assessed the prognostic significance of receptor status relative to the Nottingham prognostic index (NPI). The ER-alpha and ER-beta status was also determined by immunohistochemistry using previously published and 'in-house' scoring systems.Results. Using RT-PCR analysis, 46 tumours were hormone receptor positive (ER+) with 42 displaying ER-alpha predominance. Comparison with immunohistochemistry demonstrated 44/53 (ER-alpha) and 27/50 (ER-beta) concordance rates. There was no significant difference in the NPI between ER-alpha and ER-beta predominant cohorts or between ER+ and ER- cohorts.Conclusion. This study identifies the existence of a subgroup of ER+ patients in whom Tamoxifen therapy may be inappropriate and has significant implications for adjuvant therapy of primary breast cancer. (C) 2004 Elsevier Ltd. All rights reserved.
10.1016/j.ejso.2004.02.010
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