Peer-Reviewed Journal Details
Mandatory Fields
Thomas, D,Fontana, G,Chen, XZ,Sanz-Nogues, C,Zeugolis, DI,Dockery, P,O'Brien, T,Pandit, A
2014
October
Biomaterials
A shape-controlled tuneable microgel platform to modulate angiogenic paracrine responses in stem cells
Published
()
Optional Fields
Customised ECM matrices Microgels Mesenchymal stem cells Cell-factories Angiogenesis TISSUE ENGINEERING APPLICATIONS EXTRACELLULAR-MATRIX MOUSE MODEL MOLECULAR-MECHANISMS LIMB ISCHEMIA HYDROGELS GROWTH MIGRATION MEDICINE FATE
35
31
8757
8766
Development of cell delivery platforms have been driven based on an empirical cytoprotective design. While cell-matrix and cell cell interactions that influence biochemical effects beyond survival has been limited and overshadowed in an effort to incrementally improve biomimicking properties of the tissue-engineered constructs. Here we demonstrate fabrication of a shape controlled 3D type-I collagen-based microgel platform that can be tuned to modulate angiogenic paracrine- 'angiocrine' responses of human mesenchymal stem cells (hMSCs). Furthermore, these microgels were characterized as a 3D cell culture tool to assess optimal biological response as a function of cell-matrix and cell-cell interactions. Finally, optimised hMSC embedded microgels were shown to induce vascular repair and functional improvement in vivo in a mouse model of hind-limb ischemia. The approach described here in designing a tuneable cell delivery platform using naturally occurring extracellular matrix molecules highlights the need for highly customised matrices with an array of self-assembling proteins that dictate specific cell function resembling the native tissue of interest for repair. (C) 2014 Elsevier Ltd. All rights reserved.
10.1016/j.biomaterials.2014.06.053
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