Peer-Reviewed Journal Details
Mandatory Fields
Mulvihill, JJ,Cunnane, EM,McHugh, SM,Kavanagh, EG,Walsh, SR,Walsh, MT
2013
January
Mechanical, biological and structural characterization of in vitro ruptured human carotid plaque tissue
Published
()
Optional Fields
9
9027
9035
Recent experimental studies performed on human carotid plaques have focused on mechanical characterization for the purpose of developing material models for finite-element analysis without quantifying the tissue composition or relating mechanical behaviour to preoperative classification. This study characterizes the mechanical and biological properties of 25 human carotid plaques and also investigates the common features that lead to plaque rupture during mechanical testing by performing circumferential uniaxial tests, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) on each specimen to relate plaque composition to mechanical behaviour. Mechanical results revealed large variations between plaque specimen behaviour with no correlation to preoperative ultrasound prediction. However, FTIR classification demonstrated a statistically significant relationship between stress and stretch values at rupture and the level of calcification (P = 0.002 and P = 0.009). Energy-dispersive X-ray spectroscopy was carried out to confirm that the calcium levels observed using FTIR analysis were accurate. This work demonstrates the potential of FTIR as an alternative method to ultrasound forpredicting plaque mechanical behaviour. SEM imaging at the rupture sites of each specimen highlighted voids created by the nodes of calcifications in the tissue structure which could lead to increased vulnerability of the plaque. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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