Peer-Reviewed Journal Details
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Satyam, A,Subramanian, GS,Raghunath, M,Pandit, A,Zeugolis, DI
2014
March
Journal Of Tissue Engineering And Regenerative Medicine
In vitro evaluation of Ficoll-enriched and genipin-stabilised collagen scaffolds
Published
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Optional Fields
cell attachment and growth Ficoll mechanical and thermal properties genipin collagen films TISSUE ENGINEERING APPLICATIONS CROSS-LINKING REAGENT RAT TAIL TENDON DYE-MEDIATED PHOTOOXIDATION WATER-SOLUBLE CARBODIIMIDE MECHANICAL-PROPERTIES BIOLOGICAL TISSUE EXTRACELLULAR-MATRIX STEM-CELLS FIBERS
8
233
241
Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll (R) scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra-structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications. Copyright (c) 2012 John Wiley & Sons, Ltd.
10.1002/term.1522
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