Conference Publication Details
Mandatory Fields
Ana V. Linares, Robert Gorkin III, Barry Glynn, Neus Godino, Nicola Miller, Michael Kerin,Thomas Barry, Terry Smith, and Jens Ducrée
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer
2011
October
Published
0
()
Optional Fields
Microhomogenizer centrifugal microfluidics miRNA extraction whole blood
1460
1462
Seattle, Washington, USA
02-OCT-11
06-OCT-11
In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent®(TRI) to pro-vide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase sepa-rated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent re-sistant cyclo olefin polymer (Zeonor®) disc was used in this case.
Grant Details
Science Foundation Ireland (SFI)
Publication Themes