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McCoy, GR,Touzet, N,Fleming, GTA,Raine, R
2013
October
Environmental Science And Pollution Research
An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters
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Microarrays Hierarchical probes Ribosomal RNA HABs Environmental monitoring Cell concentrations REAL-TIME PCR ALEXANDRIUM-MINUTUM DINOPHYCEAE IN-SITU HYBRIDIZATION RIBOSOMAL-RNA OLIGONUCLEOTIDE PROBES HETEROSIGMA-AKASHIWO GENETIC DIVERSITY IDENTIFICATION FLUORESCENT QUANTIFICATION
20
6751
6764
The applicability of microarrays to monitor harmful algae across a broad range of ecological niches and toxic species responsible for harmful algal events has been one of the key tasks in the EU Seventh Framework Programme (FP7)-funded Microarrays for the Detection of Toxic Algae project. The technique has a strong potential for improving speed and accuracy of the identification of harmful algae and their toxins to assist monitoring programmes. Water samples were collected from a number of coastal sites around Ireland, including several that are used in the Irish National Phytoplankton and Biotoxin Monitoring Programme. Ribosomal RNA was extracted from filtered field samples, labelled with a fluorescent dye, and hybridised to probes spotted in a microarray format on a glass slide. The fluorescent signal intensity of the hybridisation to > 120 probes on the chip was analysed and compared with actual field counts. There was a general agreement between cell counts and microarray signal. Results are presented for field samples taken from a range of stations along the Irish coastline known for harmful algal events during the first field trial (July 2009-April 2010).
DOI 10.1007/s11356-012-1294-1
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