Peer-Reviewed Journal Details
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Gupta S, Giricz Z, Natoni A, Donnelly N, Deegan S, Szegezdi E, Samali A
2012
November
Febs Letters
NOXA contributes to the sensitivity of PERK-deficient cells to ER stress.
Published
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Optional Fields
586
22
4023
4030
PKR-like ER kinase (PERK) deficient mouse embryonic fibroblasts (MEFs) are hypersensitive to ER stress-induced apoptosis. However, the molecular determinants of increased sensitivity of PERK(-/-) MEFs are not clearly understood. Here we show that induction of several Unfolded Protein Response (UPR) target genes is attenuated in PERK(-/-) MEFs. We also report elevated expression of the BH3-only protein, NOXA in PERK(-/-) MEFs. Further, shRNA-mediated knockdown of NOXA rescued the hypersensitivity of PERK(-/-) MEFs to ER stress-induced apoptosis. Taken together our results suggest that compromised induction of UPR and increased NOXA expression contributes to hypersensitivity of PERK(-/-) MEFs to ER stress-induced apoptosis.
10.1016/j.febslet.2012.10.002
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