Peer-Reviewed Journal Details
Mandatory Fields
Conghaile, PO,Kamireddy, S,MacAodha, D,Kavanagh, P,Leech, D
2013
April
Analytical And Bioanalytical Chemistry
Mediated glucose enzyme electrodes by cross-linking films of osmium redox complexes and glucose oxidase on electrodes
Published
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Optional Fields
Biosensors Electrochemical sensor Enzymes Glucose oxidase Osmium Enzymatic fuel cell BIOFUEL CELLS PHYSIOLOGICAL BUFFER CARBON ELECTRODES POLYMER STABILITY RUTHENIUM(II) PERFORMANCE SERIES
405
3807
3812
Here, we report on a novel, versatile approach for the preparation of mediated enzyme electrodes, demonstrated using cross-linked films of glucose oxidase and a range of functionalised osmium complexes on graphite electrodes. Response of enzyme electrodes are optimised by evaluation of glucose response as a function of variation in ratios of [Os (2,2'-bipyridine)(2)(4-aminomethyl pyridine)Cl](+) redox mediator, polyallylamine support and glucose oxidase enzyme cross-linked using a di-epoxide reagent in films on graphite. Lowering of the redox potential required to mediate glucose oxidation is achieved by synthesis of complexes using (4,4'-dimethyl- 2,2'-bipyridine) or (4,4'-dimethoxy-2,2'-bipyridine) as a ligand instead of (2,2'-bipyridine). Enzyme electrodes prepared using the complexes based on dimethoxyor dimethyl-substituted bipyridines provide glucose oxidation current densities of 30 and 70 mu Acm(-2) at 0.2 and 0.35 V applied potential compared to 120 mu Acm(-2) at 0.45 V for the initial enzyme electrode, under pseudo-physiological conditions in 5 mM glucose, with stability of signals proving inadequate for long-term operation. Current output and stability may be improved by selection of alternate anchoring and cross-linking methodology, to provide enzyme electrodes capable for application to long-term glucose biosensors and anodes in enzymatic fuel cells.
DOI 10.1007/s00216-012-6628-9
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