Raman spectroscopy is a versatile vibrational spectroscopy technique which can provide extensive information about molecular composition. In the small molecule Active Pharmaceutical Ingredient domain, the method is widely used for raw materials and process applications. However, in the Biological API (bAPI) domain there has been much less application of the method. The primary reason for this is the fact that the bAPI domain is an inherently much more complex system. Most of the materials which one needs to characterise in terms of compositional variance or for quantitative concentration measurement are very complex materials. This is particularly true of bioprocess broths, cell culture media and more exceptionally so in the case of hydrosylates generated from yeast and meat protein. Here we show how Raman spectroscopy using 785 nm excitation can be used for the quantitative analysis of complex chemically defined cell culture media solutions and bioprocess broths. We also show how the longer wavelength 993 nm excitation allows for the facile analysis of the very complex, solid hydrosylates used as a component of media employed for mammalian cell and E-coli based fermentation.