Cell culture media (CCM) used in industrial
mammalian cell culture are complex
aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in efficient biopharmaceutical manufacturing. We are exploring the use
of rapid “holistic” analytical methods that
can be used for routine CCM screening.
The application of rapid fluorescence
and Raman spectroscopic techniques
to the routine media analysis for Chinese
Hamster Ovary cell-based manufacture
was investigated. These robust and
inexpensive methods can be used to
identify compositional changes and
ultimately predict the efficacy of individual
fed batch media in terms of bioreactor titre.
This is achieved through the application of
a diverse range of chemometric methods.
This ability to observe compositional
changes and predict end titre before
media use has enormous potential and
should permit the effective elimination of
one of the major process variables leading
to more consistent product quality and
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