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Conroy, PC,Saladino, C,Dantas, TJ,Lalor, P,Dockery, P,Morrison, CG
2012
October
Cell Cycle
C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis
Published
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Optional Fields
centrosome DNA damage response kizuna C-NAP1 NEK2 CELL-CYCLE CENTROSOME AMPLIFICATION MOTHER CENTRIOLES PROTEIN-KINASE PRIMARY CILIUM BETA-CATENIN G2 PHASE DUPLICATION NEK2 SEPARATION
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Cilia are found on most human cells and exist as motile cilia or non-motile primary cilia. Primary cilia play sensory roles in transducing various extracellular signals, and defective ciliary functions are involved in a wide range of human diseases. Centrosomes are the principal microtubule-organizing centers of animal cells and contain two centrioles. We observed that DNA damage causes centriole splitting in non-transformed human cells, with isolated centrioles carrying the mother centriole markers CEP 170 and ninein but not kizuna or cenexin. Loss of centriole cohesion through siRNA depletion of C-NAP1 or rootletin increased radiation-induced centriole splitting, with C-NAP1-depleted isolated centrioles losing mother markers. As the mother centriole forms the basal body in primary cilia, we tested whether centriole splitting affected ciliogenesis. While irradiated cells formed apparently normal primary cilia, most cilia arose from centriolar clusters, not from isolated centrioles. Furthermore, C-NAP1 or rootletin knockdown reduced primary cilium formation. Therefore, the centriole cohesion apparatus at the proximal end of centrioles may provide a target that can affect primary cilium formation as part of the DNA damage response.
DOI 10.4161/cc.21986
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