Peer-Reviewed Journal Details
Mandatory Fields
Verfaillie, T,Rubio, N,Garg, AD,Bultynck, G,Rizzuto, R,Decuypere, JP,Piette, J,Linehan, C,Gupta, S,Samali, A,Agostinis, P
2012
November
Cell Death And Differentiation
PERK is required at the ER-mitochondrial contact sites to convey apoptosis after ROS-based ER stress
Published
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Optional Fields
PERK mitochondria-associated membranes ROS ER stress apoptosis photodynamic therapy ENDOPLASMIC-RETICULUM STRESS HYDROGEN-PEROXIDE CYTOCHROME-C CELL-DEATH HYPERICIN CANCER PROTEINS PATHWAY ACTIVATION RESISTANCE
19
1880
1891
Endoplasmic reticulum stress is emerging as an important modulator of different pathologies and as a mechanism contributing to cancer cell death in response to therapeutic agents. In several instances, oxidative stress and the onset of endoplasmic reticulum (ER) stress occur together; yet, the molecular events linking reactive oxygen species (ROS) to ER stress-mediated apoptosis are currently unknown. Here, we show that PERK (RNA-dependent protein kinase (PKR)-like ER kinase), a key ER stress sensor of the unfolded protein response, is uniquely enriched at the mitochondria-associated ER membranes (MAMs). PERK-/- cells display disturbed ER morphology and Ca2+ signaling as well as significantly weaker ER-mitochondria contact sites. Re-expression of a kinase-dead PERK mutant but not the cytoplasmic deletion mutant of PERK in PERK-/- cells re-establishes ER-mitochondria juxtapositions and mitochondrial sensitization to ROS-mediated stress. In contrast to the canonical ER stressor thapsigargin, during ROS-mediated ER stress, PERK contributes to apoptosis twofold by sustaining the levels of pro-apoptotic C/EBP homologous protein (CHOP) and by facilitating the propagation of ROS signals between the ER and mitochondria through its tethering function. Hence, this study reveals an unprecedented role of PERK as a MAMs component required to maintain the ER-mitochondria juxtapositions and propel ROS-mediated mitochondrial apoptosis. Furthermore, it suggests that loss of PERK may cause defects in cell death sensitivity in pathological conditions linked to ROS-mediated ER stress. Cell Death and Differentiation (2012) 19, 1880-1891; doi:10.1038/cdd.2012.74; published online 15 June 2012
DOI 10.1038/cdd.2012.74
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