Peer-Reviewed Journal Details
Mandatory Fields
Mary Garvey, M., Clifford E., O'Reilly E., Rowan N.J.
2013
January
Journal Of Parasitology
Efficacy of Using Harmless Bacillus Endospores to Estimate the Inactivation of Cryptosporidium parvum Oocysts in Water
Published
()
Optional Fields
99
3
448
452
The need to use complex in vitro cell culture, expensive equipment, and highly-trained technicians that are available only to specialist laboratories has significantly limited studies assessing the potential of pulsed UV light (PUV) to inactivate the waterborne parasite Cryptosporidium parvum in drinking water. This constitutes the first study to report on the use of different non-pathogenic Bacillus endospores as potential surrogate organisms to indicate the PUV inactivation performance of a C. parvum oocyst suspended in water. Findings showed that PUV effectively inactivated approximately 5 log10 CFU/ml Bacillus megaterium and Bacillus pumilus endospores suspended in water at a UV dose of 9.72 μJ/cm2 that also inactivated statistically similar levels of C. parvum oocysts (P < 0.05), as determined by combined in vitro HCT-8 cell culture and quantitative PCR. Specifically, this study demonstrated that B. megaterium exhibited greater or similar PUV-inactivation kinetic data compared to that of si...
0022-3395
https://www.jstor.org/stable/41982437
10.1645/12-48.1
Grant Details
Environmental Protection Agency (EPA)
2011-W-MS-5
Publication Themes
Environment, Marine and Energy