Peer-Reviewed Journal Details
Mandatory Fields
Mathew, A,Cao, HL,Collin, E,Wang, WX,Pandit, A
International Journal Of Pharmaceutics
Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector
Optional Fields
2-(Dimethylamino) ethyl methacrylate Deactivation enhanced atom transfer radical polymerisation Gene therapy RADICAL POLYMERIZATION DNA DELIVERY THERAPY SYSTEMS POLYETHYLENIMINE PERSPECTIVES MICELLES POLYMERS MEDICINE CARRIER
A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine) dendrimer (dPAMAM; commercial name: SuperFect (R)) in fibroblasts and adipose tissue derived stem cells (ADSCs). (c) 2012 Elsevier B.V. All rights reserved.
DOI 10.1016/j.ijpharm.2012.05.010
Grant Details
Publication Themes