Peer-Reviewed Journal Details
Mandatory Fields
Holladay, CA,Duffy, AM,Chen, XZ,Sefton, MV,O'Brien, TD,Pandit, AS
2012
February
Biomaterials
Recovery of cardiac function mediated by MSC and interleukin-10 plasmid functionalised scaffold
Published
()
Optional Fields
Anti-inflammatory gene transfer Mesenchymal stem cells Cardiac tissue engineering Scaffold Interleukin-10 ACUTE MYOCARDIAL-INFARCTION STEM-CELL TRANSPLANTATION GENE DELIVERY IN-VIVO ISCHEMIA/REPERFUSION INJURY MACROPHAGE PHENOTYPE VENTRICULAR-FUNCTION HEART SURVIVAL THERAPY
33
1303
1314
Stem cell transplantation has been suggested as a treatment for myocardial infarction, but clinical studies have yet to demonstrate conclusive, positive effects. This may be related to poor survival of the transplanted stem cells due to the inflammatory response following myocardial infarction. To address this, a scaffold-based stem cell delivery system was functionalised with anti-inflammatory plasmids (interleukin-10) to improve stem cell retention and recovery of cardiac function. Myocardial infarction was induced and these functionalised scaffolds were applied over the infarcted myocardium. Four weeks later, stem cell retention, cardiac function, remodelling and inflammation were quantified. Interleukin-10 gene transfer improved stem cell retention by more than five-fold and the hearts treated with scaffold, stem cells and interleukin-10 had significant functional recovery compared to the scaffold control (scaffold: -10+/-7%, scaffold, interleukin-10 and stem cells: +7 +/-6%). This improved function was associated with increased infarcted wall thickness and increased ratios of collagen type III/type I, decreased cell death, and a change in macrophage markers from mainly cytotoxic in the scaffold group to mainly regulatory in scaffold, stem cells and interleukin-10 group. Thus, treatment of myocardial infarction with stem cells and interleukin-10 gene transfer significantly improved stem cell retention and ultimately improved overall cardiac function. (C) 2011 Elsevier Ltd. All rights reserved.
DOI 10.1016/j.biomaterials.2011.10.019
Grant Details
Publication Themes