Peer-Reviewed Journal Details
Mandatory Fields
Zafar, MN,Beden, N,Leech, D,Sygmund, C,Ludwig, R,Gorton, L
2012
February
Analytical And Bioanalytical Chemistry
Characterization of different FAD-dependent glucose dehydrogenases for possible use in glucose-based biosensors and biofuel cells
Published
()
Optional Fields
Glucose biosensor FAD Glucose dehydrogenase Os-polymer Deglycosylation Biofuel cell DIRECT ELECTRON-TRANSFER ENZYMATIC FUEL-CELLS GRAPHITE-ELECTRODES CELLOBIOSE DEHYDROGENASE AMPEROMETRIC BIOSENSORS ADENINE-DINUCLEOTIDE CATALYTIC-OXIDATION CARBON ELECTRODES REDOX POLYMERS OXIDASE
402
2069
2077
In this study, different flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenases (FADGDHs) were characterized electrochemically after "wiring" them with an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)(2)(PVI)(10)Cl](+) on graphite electrodes. One tested FADGDH was that recently discovered in Glomerella cingulata (GcGDH), another was the recombinant form expressed in Pichia pastoris (rGcGDH), and the third was a commercially available glycosylated enzyme from Aspergillus sp. (AspGDH). The performance of the Os-polymer "wired" GDHs on graphite electrodes was tested with glucose as the substrate. Optimal operational conditions and analytical characteristics like sensitivity, linear ranges and current density of the different FADGDHs were determined. The performance of all three types of FADGDHs was studied at physiological conditions (pH 7.4). The current densities measured at a 20 mM glucose concentration were 494 +/- 17, 370 +/- 24, and 389 +/- 19 mu A cm(-2) for GcGDH, rGcGDH, and AspGDH, respectively. The sensitivities towards glucose were 2.16, 1.90, and 1.42 mu A mM(-1) for GcGDH, rGcGDH, and AspGDH, respectively. Additionally, deglycosylated rGcGDH (dgrGcGDH) was investigated to see whether the reduced glycosylation would have an effect, e.g., a higher current density, which was indeed found. GcGDH/Os-polymer modified electrodes were also used and investigated for their selectivity for a number of different sugars.
DOI 10.1007/s00216-011-5650-7
Grant Details
Publication Themes