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Kilcoyne, M,Shashkov, AS,Senchenkova, SA,Knirel, YA,Vinogradov, EV,Radziejewska-Lebrecht, J,Galimska-Stypa, R,Savage, AV
Structural investigation of the O-specific polysaccharides of Morganella morganii consisting of two higher sugars
CARBOHYDRATE RESEARCH
2002
October
Published
1
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Morganella morganii bacterial polysaccharide structure lipopolysaccharide branched monosaccharide 5,7-diamino-3,5,7,9-tetra-deoxy-L-glycero-D-galacto-non-2-ulosonic acid shewanellose 8-epilegionaminic acid ACID LIPOPOLYSACCHARIDE CHAIN
1697
1702
The lipopolysaccharide of the bacterium Morganella morganii (strain KF 1676, RK 4222) yielded two polysaccharides, PS1 and PS2, when subjected to mild acid degradation followed by GPC. The polysaccharides were studied by H-1 and C-13 NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, H-1,C-13 HMQC, and HMBC experiments. Each polysaccharide was found to contain a disaccharide repeating unit consisting of two higher sugars, 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-L-glycero -D-galacto-non-2-ulosonic acid (a derivative of 8-epilegionaminic acid, 8eLeg5Am7Ac) and 2-acetamido-4-C-(3'-carboxamide-2',2'-dihydroxypropyl)-2,6-dideoxy-D-galactose (shewanellose, She). The two polysaccharides differ only in the ring size of shewanellose and have the following structures:PS1 --> 8)-alpha-8eLegp5Am7Ac-(2 --> 3)-beta-Shef-(1 -->PS2 --> 8)-alpha-8eLegp5Am7Ac-(2 --> 3)-beta-Shep-(1 -->Shewanellose has been previously identified in a phenol-soluble polysaccharide from Shewanella putrefaciens A6, which shows a close structural similarity to PS2. (C) 2002 Elsevier Science Ltd. All rights reserved.
PII S0008-6215(02)00181-7
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