Peer-Reviewed Journal Details
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Duffy, AM,O'Doherty, AM,O'Brien, T,Strappe, PM
2005
October
Gene Therapy
Purification of adenovirus and adeno-associated virus: Comparison of novel membrane-based technology to conventional techniques
Published
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Optional Fields
adenovirus adeno-associated virus purification ion exchange ANION-EXCHANGE HPLC COLUMN CHROMATOGRAPHY GENE-THERAPY SIALIC-ACID VECTORS TYPE-5 PARTICLES BINDING VIRION TOOLS
62
72
Adenovirus (Ad) and Adeno-associated virus (AAV) are efficient gene delivery systems; manipulation of the wild-type genome allows their use as vectors for the overexpression of desirable transgenes. Generation and purification of such viral vectors can be labour intensive, costly and require specialized equipment, but a new generation of membrane-mediated ion exchange kits for purification of recombinant virus may facilitate this process. Here, we examine the yields, transgene expression and purity of preparations of Ad and AAV purified using commercially available kits in comparison to other established techniques for purification of recombinant viral vectors. We demonstrate comparable results for Ad and AAV respectively in all parameters investigated, with a substantial reduction in purification time for the kit-based technology. Such approaches are attractive methods for small-scale purification of recombinant Ad and AAV viral vectors.
DOI 10.1038/sj.gt.3302616
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