Peer-Reviewed Journal Details
Mandatory Fields
Lopez, JC;Porca, E;Collins, G;Clifford, E;Quijano, G;Munoz, R
2019
April
Waste Management
Ammonium influences kinetics and structure of methanotrophic consortia
Published
Optional Fields
METHANE OXIDATION BIODEGRADATION KINETICS OXIDIZING BACTERIA RICE FIELD CH4 BIOFILTRATION CULTIVATION POPULATION INHIBITION PARAMETERS
89
345
353
The literature is conflicted on the influence of ammonium on the kinetics and microbial ecology of methanotrophy. In this study, methanotrophic cultures were enriched, under ammonium concentrations ranging from 0 to 200 mM, from an inoculum comprising leachate and top-cover soil from a landfill. Specific CH4 biodegradation rates were highest (7.8 x 10(-4) +/- 6.0 x 10(-5)gCH(4)g(x)(-1)h(-1)) in cultures enriched at 4 mM NH4+, which were mainly dominated by type II methanotrophs belonging to Methylocystis spp. Lower specific CH4 oxidation rates (average values of 1.8-3.6 x 10(-4)gCH(4)g(x)(-1)h(-1)) were achieved by cultures enriched at higher NH4+ concentrations (20 and 80 mM), and had higher affinity for CH4 compared to 4 mM enrichments. These lower affinities were attributed to lower diversity dominated by type I methanotrophs, of the Methylosarcina, Methylobacter and Methylomicrobium genera, encountered with increasing concentrations of NH4+. The study indicates that CH4 oxidation biotechnologies applied at low NH4+ concentrations can support efficient abatement of CH4 and high diversity of methanotrophic consortia, whilst enriching type II methanotrophs. (C) 2019 Elsevier Ltd. All rights reserved.
0956-053X
10.1016/j.wasman.2019.04.028
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