Peer-Reviewed Journal Details
Mandatory Fields
Chang, KH;Miller, N;Kheirelseid, EAH;Lemetre, C;Ball, GR;Smith, MJ;Regan, M;McAnena, OJ;Kerin, MJ
2011
November
International Journal Of Colorectal Disease
MicroRNA signature analysis in colorectal cancer: identification of expression profiles in stage II tumors associated with aggressive disease
Published
Altmetric: 1WOS: 75 ()
Optional Fields
ARTIFICIAL NEURAL-NETWORKS COLON-CANCER DUKES-B ADJUVANT CHEMOTHERAPY CIRCULATING MICRORNAS PLUS IRINOTECAN BREAST-CANCER C-MYC GENE CLASSIFICATION
26
1415
1422
Purpose Colorectal cancer (CRC) is a clinically diverse disease whose molecular etiology remains poorly understood. The purpose of this study was to identify miRNA expression patterns predictive of CRC tumor status and to investigate associations between microRNA (miRNA) expression and clinicopathological parameters.Methods Expression profiling of 380 miRNAs was performed on 20 paired stage II tumor and normal tissues. Artificial neural network (ANN) analysis was applied to identify miRNAs predictive of tumor status. The validation of specific miRNAs was performed on 102 tissue specimens of varying stages.Results Thirty-three miRNAs were identified as differentially expressed in tumor versus normal tissues. ANN analysis identified three miRNAs (miR-139-5p, miR-31, and miR-1792 cluster) predictive of tumor status in stage II disease. Elevated expression of miR-31 (p= 0.004) and miR-139-5p (p< 0.001) and reduced expression of miR-143 (p= 0.016) were associated with aggressive mucinous phenotype. Increased expression of miR-10b was also associated with mucinous tumors (p= 0.004). Furthermore, progressively increasing levels of miR-10b expression were observed from T1 to T4 lesions and from stage I to IV disease.Conclusion Association of specific miRNAs with clinicopathological features indicates their biological relevance and highlights the power of ANN to reliably predict clinically relevant miRNA biomarkers, which it is hoped will better stratify patients to guide adjuvant therapy.
0179-1958
10.1007/s00384-011-1279-4
Grant Details
Publication Themes