Peer-Reviewed Journal Details
Mandatory Fields
Pollok, S;Bauerschmidt, C;Sanger, J;Nasheuer, HP;Grosse, F
2007
July
Febs Journal
Human Cdc45 is a proliferation-associated antigen
Published
Altmetric: 3WOS: 38 ()
Optional Fields
EUKARYOTIC DNA-REPLICATION CYCLIN-DEPENDENT KINASES XENOPUS EGG EXTRACTS CELL-CYCLE S-PHASE POLYMERASE-ALPHA MINICHROMOSOME MAINTENANCE MAMMALIAN CDC6 MCM PROTEINS COMPLEX
274
3669
3684
Cell division cycle protein 45 (Cdc45) plays a critical role in DNA replication to ensure that chromosomal DNA is replicated only once per cell cycle. We analysed the expression of human Cdc45 in proliferating and nonproliferating cells. Our findings show that Cdc45 protein is absent from long-term quiescent, terminally differentiated and senescent human cells, although it is present throughout the cell cycle of proliferating cells. Moreover, Cdc45 is much less abundant than the minichromosome maintenance (Mcm) proteins in human cells, supporting the concept that origin binding of Cdc45 is rate limiting for replication initiation. We also show that the Cdc45 protein level is consistently higher in human cancer-derived cells compared with primary human cells. Consequently, tumour tissue is preferentially stained using Cdc45-specific antibodies. Thus, Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology.
1742-464X
10.1111/j.1742-4658.2007.05900.x
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