Peer-Reviewed Journal Details
Mandatory Fields
Ding, SM;Jia, F;Xu, D;Sun, Q;Zhang, L;Fan, CX;Zhang, CS
2011
November
Environmental Science & Technology
High-Resolution, Two-Dimensional Measurement of Dissolved Reactive Phosphorus in Sediments Using the Diffusive Gradients in Thin Films Technique in Combination with a Routine Procedure
Published
WOS: 35 ()
Optional Fields
IN-SITU MEASUREMENT MARINE-SEDIMENTS PERFORMANCE-CHARACTERISTICS SIMULTANEOUS RELEASE NATURAL-WATERS MICROBIAL MAT TRACE-METALS SULFIDE DGT PHOSPHATE
45
9680
9686
Dissolved reactive phosphorus (DRP) is the most available P form in sediments and often directly controls phytoplankton blooms in aquatic systems. In this study, a novel procedure was developed for two-dimensional (2D) measurement of DRP in sediments at a spatial resolution of 0.45 mm using the diffusive gradients in thin films (DGT) technique with a revised high-capacity binding phase (Zr oxide gel). This procedure involves DGT uptake of P in sediments, 2D slicing of the binding gel on a 0.45 x 0.45-mm grid system, elution of P from each gel square with 1 MNaOH, and microcolorimetric determination of DRP in each eluted solution using 384-microwell plates. Measurements of DRP via this procedure were tested in homogeneous solutions and sediments and produced an acceptable error (<20% relative standard deviation) for the analysis once the accumulated mass of P in each gel square reached 1.2 mu g cm(-2). This method was successfully applied to produce 2D images of the DRP distribution in sediments with and without the influence of tubificid worm bioturbation, revealing a much more pronounced and localized impact from tubificid worms than that found using a one-dimensional measurement of pore water DRP concentrations at 1-cm resolution.
0013-936X
10.1021/es202785p
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