Peer-Reviewed Journal Details
Mandatory Fields
Calderon, L;Collin, E;Velasco-Bayon, D;Murphy, M;O'Halloran, D;Pandit, A
2010
July
European Cells & Materials
TYPE II COLLAGEN-HYALURONAN HYDROGEL - A STEP TOWARDS A SCAFFOLD FOR INTERVERTEBRAL DISC TISSUE ENGINEERING
Published
WOS: 74 ()
Optional Fields
NUCLEUS PULPOSUS CELLS LOW-BACK-PAIN IN-VITRO LINK-PROTEIN ALGINATE CULTURES ANNULUS FIBROSUS GENE-EXPRESSION ACID MATRIX DIFFERENTIATION
20
134
148
Intervertebral disc regeneration strategies based on stern cell differentiation in combination with the design of functional scaffolds is an attractive approach towards repairing/regenerating the nucleus pulposus. The specific aim of this study was to optimise a composite hydrogel composed of type II collagen and hyaluronic acid (HA) as a carrier for mesenchymal stem cells. Hydrogel stabilisation was achieved by means of 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) cross-linking. Optimal hydrogel properties were determined by investigating different concentrations of EDC (8mM, 24mM and 48mM). Stable hydrogels were obtained independent of the concentration of carbodiimide used. The hydrogels crosslinked by the lowest concentration of EDC (8mM) demonstrated high swelling properties. Additionally, improved proliferation of seeded rat mesenchymal stem cells (rMSCs) and hydrogel stability levels in culture were observed with this 8mM cross-linked hydrogel. Results from this study indicate that EDC/NHS (8mM) cross-linked type II collagen/HA hydrogel was capable of supporting viability of rMSCs, and furthermore their differentiation into a chondrogenic lineage. Further investigations should be conducted to determine its potential as scaffold for nucleus pulposus regeneration/repair.
1473-2262
Grant Details
Publication Themes