Peer-Reviewed Journal Details
Mandatory Fields
Butler, RK;Ford, GK;Hogan, M;Roche, M;Doyle, KM;Kelly, JP;Kendall, DA;Chapman, V;Finn, DP
2012
January
Journal Of Psychopharmacology
Fear-induced suppression of nociceptive behaviour and activation of Akt signalling in the rat periaqueductal grey: role of fatty acid amide hydrolase
Published
WOS: 14 ()
Optional Fields
STRESS-INDUCED ANALGESIA ENDOGENOUS CANNABINOID SYSTEM ROSTRAL VENTROMEDIAL MEDULLA CONDITIONED ANALGESIA ENDOCANNABINOID SYSTEM OPIOID ANALGESIA FAAH INHIBITOR MODULATION PAIN RECEPTORS
26
83
91
The endocannabinoid system regulates nociception and aversion and mediates fear-conditioned analgesia (FCA). We investigated the effects of the fatty acid amide hydrolase (FAAH) inhibitor URB597, which inhibits the catabolism of the endocannabinoid anandamide and related N-acylethanolamines, on expression of FCA and fear and pain related behaviour per se in rats. We also examined associated alterations in the expression of the signal transduction molecule phospho-Akt in the periaqueductal grey (PAG) by immunoblotting. FCA was modelled by assessing formalin-evoked nociceptive behaviour in an arena previously paired with footshock. URB597 (0.3 mg/kg, i.p.) enhanced FCA and increased fear-related behaviour in formalin-treated rats. Conditioned fear per se in non-formalin-treated rats was associated with increased expression of phospho-Akt in the PAG. URB597 reduced the expression of fear-related behaviour in the early part of the trial, an effect that was accompanied by attenuation of the fear-induced increase in phospho-Akt expression in the PAG. Intra-plantar injection of formalin also reduced the fear-induced increase in phospho-Akt expression. These data provide evidence for a role of FAAH in FCA, fear responding in the presence or absence of nociceptive tone, and fear-evoked increases in PAG phospho-Akt expression. In addition, the results suggest that fear-evoked activation of Akt signalling in the PAG is abolished in the presence of nociceptive tone.
0269-8811
10.1177/0269881111413823
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