Peer-Reviewed Journal Details
Mandatory Fields
Bialek, K;Kumar, A;Mahony, T;Lens, PNL;O' Flaherty, V
2012
November
Microbial Biotechnology
Microbial community structure and dynamics in anaerobic fluidized-bed and granular sludge-bed reactors: influence of operational temperature and reactor configuration
Published
WOS: 15 ()
Optional Fields
WASTE-WATER TREATMENT POLYMERASE-CHAIN-REACTION NEIGHBOR-JOINING METHOD REAL-TIME PCR METHANOGENIC COMMUNITIES DEGREES-C LAKE SEDIMENT PADDY SOIL HYDROGEN WASTEWATERS
5
738
752
Methanogenic community structure and dynamics were investigated in two different, replicated anaerobic wastewater treatment reactor configurations [inverted fluidized bed (IFB) and expanded granular sludge bed (EGSB)] treating synthetic dairy wastewater, during operating temperature transitions from 37 degrees C to 25 degrees C, and from 25 degrees C to 15 degrees C, over a 430-day trial. Non-metric multidimensional scaling (NMS) and moving-window analyses, based on quantitative real-time PCR data, along with denaturing gradient gel electrophoresis (DGGE) profiling, demonstrated that the methanogenic communities developed in a different manner in these reactor configurations. A comparable level of performance was recorded for both systems at 37 degrees C and 25 degrees C, but a more dynamic and diverse microbial community in the IFB reactors supported better stability and adaptative capacity towards low temperature operation. The emergence and maintenance of particular bacterial genotypes (phylum Firmicutes and Bacteroidetes) was associated with efficient protein hydrolysis in the IFB, while protein hydrolysis was inefficient in the EGSB. A significant community shift from a Methanobacteriales and Methanosaetaceae towards a Methanomicrobiales-predominated community was demonstrated during operation at 15 degrees C in both reactor configurations.
1751-7907
10.1111/j.1751-7915.2012.00364.x
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