Peer-Reviewed Journal Details
Mandatory Fields
Gundy, S;Van der Putten, W;Shearer, A;Buckton, D;Ryder, AG;Ball, M
2004
February
Physics In Medicine And Biology
The use of chloroaluminium phthalocyanine tetrasulfonate (AlPcTS) for time-delayed fluorescence imaging
Published
WOS: 5 ()
Optional Fields
TUMOR-BEARING MICE PHOTODYNAMIC THERAPY ALUMINUM PHTHALOCYANINE MURINE MODEL DIAGNOSIS SPECTROSCOPY SCATTERING PHANTOM SAMPLES CANCER
49
359
369
Phthalocyanine derivatives are currently under investigation for use in photodynamic therapy, which is a promising cancer treatment. These materials, which display preferential uptake in cancerous cells, also exhibit high fluorescence yields and can be used for tumour detection. Problems with steady-state fluorescence techniques such as excitation scatter and background autofluorescence can be eliminated by using time-resolved imaging techniques without the need for filters. A tissue phantom was assembled to test a constructed time-gated imaging system by drilling 36 wells of varying diameter and depth (10 mm to 1 mm) into a block of polymethyl methacrylate (PMMA). The system was used to record images of chloroaluminium phthalocyanine tetrasulfonate (AlPcTS) at differing concentrations in neat aqueous solvent and cell suspensions within the wells. A mixture of Intralipid (to mimic tissue scatter) and Evan's blue (to mimic tissue absorption) of depths ranging from 1 mm to 10 mm was placed on top of the PMMA block. The ensuing images were analysed using signal-to-noise ratios and contrast-detail curves. The results indicate that the time-gated imaging system can prevent background excitation scatter from distorting the fluorescence signal from a longer-lived photosensitizer without the need for filters.
0031-9155
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