Conference Publication Details
Mandatory Fields
Togashi, DM;Ryder, AG
JOURNAL OF FLUORESCENCE
Time-resolved fluorescence studies on Bovine Serum Albumin denaturation process
2006
March
Published
1
WOS: 30 ()
Optional Fields
PROTEINS BINDING DISEASE
153
160
The denaturation of Bovine Serum Albumin (BSA) by a chaotropic agent, guanidinium hydrochloride (GuH(+)Cl(-)) was studied by fluorescence lifetime analysis. The BSA was labelled with 1-anilino-8-naphthalene sulfonate (ANS) at two different molar ratios (1:1) and (1:10). The non-exponential fluorescence kinetics of the BSA-ANS complex at different stages of denaturation is analysed using three different models: a discrete tri-exponential sum, stretched exponential, and Gaussian lifetime distribution. In all cases, the fluorescence decay times decreased with protein denaturation. The results from the models show that there are at least two different binding sites located in the BSA protein with different water accessibility.
10.1007/s10895-005-0029-9
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