Peer-Reviewed Journal Details
Mandatory Fields
Curley, GF;Contreras, M;Higgins, B;O'Kane, C;McAuley, DF;O'Toole, D;Laffey, JG
2011
November
Anesthesiology
Evolution of the Inflammatory and Fibroproliferative Responses during Resolution and Repair after Ventilator-induced Lung Injury in the Rat
Published
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Optional Fields
RESPIRATORY-DISTRESS-SYNDROME END-EXPIRATORY PRESSURE HYPERCAPNIC ACIDOSIS MECHANICAL VENTILATION MATRIX METALLOPROTEINASES BACTERIAL PNEUMONIA PULMONARY INFLAMMATION SYSTEMIC SEPSIS GROWTH-FACTOR RISK-FACTOR
115
1022
1032
Background: The time course and mechanisms of resolution and repair, and the potential for fibrosis following ventilation-induced lung injury (VILI), are unclear. We sought to examine the pattern of inflammation, injury, repair, and fibrosis following VILI.Methods: Sixty anesthetized rats were subject to high-stretch; low-stretch, or sham ventilation, and randomly allocated to undergo periods of recovery of 6, 24, 48, and 96 h, and 7 and 14 days. Animals were then reanesthetized, and the extent of lung injury, inflammation, and repair determined.Results: No injury was seen following low-stretch or sham ventilation. VILI caused severe lung injury, maximal at 24 h, but largely resolved by 96 h. Arterial oxygen tension decreased from a mean (SD) of 144.8 (4.1) mmHg to 96.2 (10.3) mmHg 6 h after VILI, before gradually recovering to 131.2 (14.3) mmHg at 96 h. VILI induced an early neutrophilic alveolitis and a later lymphocytic alveolitis, followed by a monocyte/macrophage infiltration. Alveolar tumor necrosis factor-alpha, interleukin-1 beta, and transforming growth factor-beta 1 concentrations peaked at 6 h and returned to baseline within 24 h, while interleukin-10 remained increased for 48 h. VILI generated a marked but transient fibroproliferative response, which restored normal lung architecture. There was no evidence of fibrosis at 7 and 14 days.Conclusions: High-stretch ventilation caused severe lung injury, activating a transient inflammatory and fibroproliferative repair response, which restored normal lung architecture without evidence of fibrosis.
0003-3022
10.1097/ALN.0b013e31823422c9
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