Anaerobic oxidation of methane (AOM) coupled to sulfate reduction is mediated by, respectively, anaerobic methanotrophic archaea (ANME) and sulfate reducing bacteria (SRB). When a microbial community from coastal marine Lake Grevelingen sediment, containing ANME-3 as the most abundant type of ANME, was incubated under a pressure gradient (0.1-40 MPa) for 77 days, ANME-3 was more pressure sensitive than the SRB. ANME-3 activity was higher at lower (0.1, 0.45MPa) over higher (10, 20, and 40 MPa) CH4 total pressures. Moreover, the sulfur metabolism was shifted upon changing the incubation pressure: only at 0.1 MPa elemental sulfur was detected in a considerable amount and SRB of the Desulfosarcina/Desulfococcus genera were more enriched at elevated pressures than the Desulfobulbus. This study provides evidence that ANME-3 can be constrained at shallow environments (45mdepth), despite the scarce bioavailable energy, because of its pressure sensitivity. Besides, the association between ANME-3 and SRB can be steered by changing solely the incubation pressure. The ANME-3 cells present in the marine Lake Grevelingen possess high specific AOM-SR rates and thus, can be of great potential to be applied in the industry after enrichment.