Peer-Reviewed Journal Details
Mandatory Fields
Mackay, AM;Beck, SC;Murphy, JM;Barry, FP;Chichester, CO;Pittenger, MF
1998
January
Tissue Engineering
Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow
Published
WOS: 871 ()
Optional Fields
GROWTH-FACTOR-BETA II COLLAGEN CHONDROCYTE DIFFERENTIATION TGF-BETA CARTILAGE DIFFERENTIATION MONOCLONAL-ANTIBODIES MATRIX COMPONENTS SURFACE ANTIGENS THYROID-HORMONE EXPRESSION
4
415
428
In the adult human, mesenchymal stem cells (MSCs) resident in bone marrow retain the capacity to proliferate and differentiate along multiple connective tissue lineages, including cartilage. In this study, culture-expanded human MSCs (hMSCs) of 60 human donors were induced to express the morphology and gene products of chondrocytes. Chondrogenesis was induced by culturing hMSCs in micromass pellets in the presence of a defined medium that included 100 nM dexamethasone and 10 ng/ml transforming growth factor-beta(3) (TGF-beta(3)). Within 14 days, cells secreted an extracellular matrix incorporating type II collagen, aggrecan, and anionic proteoglycans. hMSCs could be further differentiated to the hypertrophic state by the addition of 50 nM thyroxine, the withdrawal of TGF-beta(3), and the reduction of dexamethasone concentration to 1 nM. Increased understanding of the induction of chondrogenic differentiation should lead to further progress in defining the mechanisms responsible for the generation of cartilaginous tissues, their maintenance, and their regeneration.
2152-4947
Grant Details
Publication Themes