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Bhattarai, S,Cassarini, C,Rene, ER,Kummel, S,Esposito, G,Lens, PNL
2018
June
Engineering In Life Sciences
Enrichment of ANME-2 dominated anaerobic methanotrophy from cold seep sediment in an external ultrafiltration membrane bioreactor
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Anaerobic methanotrophs (ANME) Anaerobic oxidation of methane (AOM) Desulfosarcina Membrane bioreactor (MBR) Sulfate reduction (SR) METHANE-OXIDIZING COMMUNITIES SULFATE-REDUCING BACTERIA KAZAN MUD VOLCANO MARINE SEDIMENT WASTE-WATER OXIDATION REDUCTION ARCHAEA GULF DIVERSITY
18
368
378
Anaerobic oxidation of methane (AOM) coupled to sulfate reduction is a microbially mediated unique natural phenomenon with an ecological relevance in the global carbon balance and potential application in biotechnology. This study aimed to enrich an AOM performing microbial community with the main focus on anaerobic methanotrophic archaea (ANME) present in sediments from the Ginsburg mud volcano (Gulf of Cadiz), a known site for AOM, in a membrane bioreactor (MBR) for 726 days at 22 (+/- 3)degrees C and at ambient pressure. The MBR was equipped with a cylindrical external ultrafiltration membrane, fed a defined medium containing artificial seawater and operated at a cross flow velocity of 0.02 m/min. Sulfide production with simultaneous sulfate reduction was in equimolar ratio between days 480 and 585 of MBR operation, whereas methane consumption was in oscillating trend. At the end of the MBR operation (day 726), the enriched biomass was incubated with C-13 labeled methane, C-13 labeled inorganic carbon was produced and the AOM rate based on C-13-inorganic carbon was 1.2 mu mol/(g(dw) d). Microbial analysis of the enriched biomass at 400 and 726 days of MBR operation showed that ANME-2 and Desulfosarcina type sulfate reducing bacteria were enriched in the MBR, which formed closely associated aggregates. The major relevance of this study is the enrichment of an AOM consortium in a MBR system which can assist to explore the ecophysiology of ANME and provides an opportunity to explore the potential application of AOM.
10.1002/elsc.201700148
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