Peer-Reviewed Journal Details
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Pereira, DR,Silva-Correia, J,Oliveira, JM,Reis, RL,Pandit, A,Biggs, MJ
2018
April
Nanomedicine-Nanotechnology Biology And Medicine
Nanocellulose reinforced gellan-gum hydrogels as potential biological substitutes for annulus fibrosus tissue regeneration
Published
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Self-gelling Nanocellulose Methacrylated gellan-gum Nanocomposite hydrogels Annulus fibrosus Intervertebral disc degeneration INTERVERTEBRAL DISC DEGENERATION CELLULOSE NANOCRYSTALS CNC LOW-BACK-PAIN MECHANICAL-PROPERTIES ENGINEERING APPLICATIONS CROSS-LINKING LUMBAR NANOCOMPOSITES COMPOSITES STRATEGIES
14
897
908
Intervertebral disc (IVD) degeneration is associated with both structural damage and aging related degeneration. Annulus fibrosus (AF) defects such as annular tears, herniation and discectomy require novel tissue engineering strategies to functionally repair AF tissue. An ideal construct will repair the AF by providing physical and biological support, facilitating regeneration. The presented strategy herein proposes a gellan gum-based construct reinforced with cellulose nanocrystals (nCell) as a biological self-gelling AF substitute. Nanocomposite hydrogels were fabricated and characterized with respect to hydrogel swelling capacity, degradation rate in vitro and mechanical properties. Rheological evaluation on the nanocomposites demonstrated the GGMA reinforcement with nCell promoted matrix entanglement with higher scaffold stiffness observed upon ionic crosslinking. Compressive mechanical tests demonstrated compressive modulus values close to those of the human AF tissue. Furthermore, cell culture studies with encapsulated bovine AF cells indicated that nanocomposite constructs promoted cell viability and a physiologically relevant cell morphology for up to fourteen days in vitro. (c) 2017 Elsevier Inc. All rights reserved.
10.1016/j.nano.2017.11.011
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