Peer-Reviewed Journal Details
Mandatory Fields
Venugopal, J,Boyle, N,Kelly, JP
2003
May
Current Science
Identification of the expression of inhibitory gamma subunit of phosphodiesterase-6 in non-retinal murine tissues
Published
()
Optional Fields
PHOTORECEPTOR CGMP PHOSPHODIESTERASE PROTEINS CELLS PDE6
84
1227
1231
Phosphodiesterases (PDEs) are enzymes that maintain the integrity of various cyclic nucleotide signaling pathways by regulating the cellular levels of cyclic nucleotide secondary messengers. PDE-6 is the only member of the phosphodiesterase family that is known to be regulated by a G-protein. The gamma-inhibitory subunit of PDE-6 facilitates the interaction between G-protein (transducin) and PDE-6 and PDEgamma is the only known molecule via which G-protein and phosphodiesterase interact. We have found the expression of PDEgamma-like transcripts and proteins in non-retinal tissues such as testis and lungs by both RT-PCR and Western blotting. We identified two main isoforms of PDEgamma, which we name rod PDEgamma and cone PDEgamma. The cone PDEgamma undergoes splicing to give rise to a short isoform, which has a truncated C-terminal. We have also shown that p14 is a mixture of rod and cone gamma, while p18 corresponds to the phosphorylated rod gamma. Our results are suggestive of a widespread physiological interaction between G-protein and phosphodiesterases.
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