Peer-Reviewed Journal Details
Mandatory Fields
Beyer, A,Hollunder, J,Nasheuer, HP,Wilhelm, T
2004
November
Molecular & Cellular Proteomics
Post-transcriptional expression regulation in the yeast Saccharomyces cerevisiae on a genomic scale
Published
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Optional Fields
MESSENGER-RNA GENE-EXPRESSION PROTEIN EXPRESSION TRANSCRIPTOME ABUNDANCE TURNOVER PATHWAY
3
1083
1092
Based on large-scale data for the yeast Saccharomyces cerevisiae (protein and mRNA abundance, translational status, transcript length), we investigate the relation of transcription, translation, and protein turnover on a genome-wide scale. We elucidate variations between different spatial cell compartments and functional modules by comparing protein-to-mRNA ratios, translational activity, and a novel descriptor for protein-specific degradation (protein half-life descriptor). This analysis helps to understand the cell's strategy to use transcriptional and post-transcriptional regulation mechanisms for managing protein levels. For instance, it is possible to identify modules that are subject to suppressed translation under normal conditions ("translation on demand"). In order to reduce inconsistencies between the datasets, we compiled a new reference mRNA abundance dataset and we present a novel approach to correct large microarray signals for a saturation bias. Accounting for ribosome density based on transcript length rather than ORF length improves the correlation of observed protein levels to translational activity. We discuss potential causes for the deviations of these correlations. Finally, we introduce a quantitative descriptor for protein degradation (protein half-life descriptor) and compare it to measured half-lives. The study demonstrates significant post-transcriptional control of protein levels for a number of different compartments and functional modules, which is missed when exclusively focusing on transcript levels.
DOI 10.1074/mcp.M400099-MCP200
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