Peer-Reviewed Journal Details
Mandatory Fields
Broderick, EP,O'Halloran, DM,Rochev, YA,Griffin, M,Collighan, RJ,Pandit, AS
Journal Of Biomedical Materials Research Part B-Applied Biomaterials
Enzymatic stabilization of gelatin-based scaffolds
Optional Fields
microbial transglutaminase gelatin scaffold hydrogel tissue engineering CROSS-LINKING PROTEINS FILMS
The definitive goal of this research is to develop protein-based scaffolds for use in soft tissue regeneration, particularly in the field of dermal healing. The premise of this investigation was to characterize the mechanical properties of gelatin cross-linked with microbial transglutaminase (mTGase) and to investigate the cytocompatibility of mTGase cross-linked gelatin. Dynamic rheological analysis revealed a significant increase in the storage modulus and thermal stability of gelatin after cross-linking with mTGase. Static, unconfined compression tests showed an increase in Young's modulus of gelatin gels after mTGase cross-linking. A comparable increase in gel strength was observed with 0.03% mTGase and 0.25% glutaraidehyde cross-linked gelatin gels. In vitro studies using 3T3 fibroblasts indicated cytotoxicity at a concentration of 0.05% mTGase after 72 h. However, no significant inhibition of cell proliferation was seen with cells grown on lower concentrations of mTGase cross-linked gelatin substrates. The mechanical improvement and cytocompatibility of mTGase crosslinked gelatin suggests mTGase has potential for use in stabilizing gelatin gels for tissueengineering applications. (C) 2004 Wiley Periodicals, Inc.
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