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Kavanagh, ET,Loughlin, JP,Herbert, KR,Dockery, P,Samall, A,Doyle, KM,Gorman, AM
2006
December
Biochemical And Biophysical Research Communications
Functionality of NGF-protected PC12 cells following exposure to 6-hydroxydopamine
Published
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Optional Fields
apoptosis catecholamines dopamine necrosis neurite outgrowth Parkinson's disease tyrosine hydroxylase NERVE GROWTH-FACTOR DOPAMINERGIC-NEURONS PARKINSONS-DISEASE SYMPATHETIC NEURONS RAT MODEL APOPTOSIS DEATH ACTIVATION PREVENTION SURVIVAL
351
890
895
6-Hydroxydopamine (6-OHDA) is often used in models of Parkinson's disease since it can selectively target and kill dopaminergic cells of the substantia nigra. In this study, pre-treatment of PC12 cells with nerve growth factor (NGF) inhibited apoptosis and necrosis by 6-OHDA, including caspase activity and lactate dehydrogenase release. Notably, cells exposed to 6-OHDA in the presence of NGF were subsequently capable of proliferation (when replated without NGF), or neurite outgrowth (with continued presence of NGF). Following 7 days growth in the presence of NGF, expression of 0111 tubulin and tyrosine hydroxylase and increased intracellular catechol-amines was detectable in PC12 cells, features characteristic of functional dopaminergic neurons. NGF-pre-treated PC12 cells retained expression of beta III-tubulin and tyrosine hydroxylase, but not catecholamine content following 6-OHDA exposure. These data indicate that NGF-protected cells maintained some aspects of functionality and were subsequently capable of proliferation or differentiation. (c) 2006 Elsevier Inc. All rights reserved.
DOI 10.1016/j.bbrc.2006.10.104
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