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Collins, G,Kavanagh, S,McHugh, S,Connaughton, S,Kearney, A,Rice, O,Carrigg, C,Scully, C,Bhreathnach, N,Mahony, T,Madden, P,Enright, AM,O'Flaherty, V
2006
May
Journal Of Environmental Science And Health Part A-Toxic/Hazardous Substances & Environmental Engineering
Accessing the black box of microbial diversity and ecophysiology: Recent advances through polyphasic experiments
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Optional Fields
microbial ecology 16S rRNA gene TRFLP DGGE FISH ecophysiology soil anaerobic sludge 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBES GRADIENT GEL-ELECTROPHORESIS IN-SITU HYBRIDIZATION COMMUNITY STRUCTURE ACTIVATED-SLUDGE DNA EXTRACTION SOIL POPULATIONS GENES
41
897
922
The microbial ecology of a range of anaerobic biological assemblages ( granular sludge) from full- and laboratory-scale wastewater treatment bioreactors, and of crop-growing and peat soils, was determined using a variety of 16S rRNA gene-based techniques, including clone library, terminal restriction fragment length polymorphism (TRFLP) and denaturing gradient gel electrophoresis (DGGE) analyses. Fluorescent in situ hybridization ( FISH) using 16S rRNA gene-targeted probes was employed to complete a "full-cycle rRNA approach" with selected biomass. Genetic fingerprinting ( TRFLP and DGGE) was effectively used to elucidate community structure-crop relationships, and to detect and monitor trends in bioreactor sludge and specific enrichment cultures of peat soil. Greater diversity was resolved within bacterial than within archaeal communities, and unexpected reservoirs of uncultured Crenarchaeota were detected in sludge granules. Advanced radiotracer incubations and micro-beta imaging were employed in conjunction with FISH to elucidate the eco-functionalism of these organisms. Crenarchaeota clusters were identified in close associated with methanogenic Archaea and both were localised with acetate uptake in biofilm structure.
DOI 10.1080/10934520600614546
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