Peer-Reviewed Journal Details
Mandatory Fields
Punchard, MA,O'Cearbhaill, ED,Mackle, JN,McHugh, PE,Smith, TJ,Stenson-Cox, C,Barron, V
2009
July
Annals Of Biomedical Engineering
Evaluation of Human Endothelial Cells Post Stent Deployment in a Cardiovascular Simulator In Vitro
Published
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Optional Fields
Stent Pre-clinical evaluation Model human artery Stent deployment in vitro Cellular response FLUID SHEAR-STRESS ADHESION MOLECULE EXPRESSION WALL MECHANICS HEMODYNAMIC FORCES INHIBITS ADHESION GENE-EXPRESSION PULSATILE FLOW CYCLIC STRAIN TIME-COURSE RESTENOSIS
37
1322
1330
Percutaneous stent implantation has revolutionized the clinical treatment of occluded arteries. Nevertheless, there is still a large unmet need to prevent re-occlusion after implantation. Consequently, a niche exists for a cost-effective pre-clinical method of evaluating novel interventional devices in human models. Therefore, the development of a coronary model artery offers tremendous potential for the treatment of endothelial cell dysfunction and restenosis. As a first step, we employ tissue-engineering principles to examine the effect of stent deployment upon endothelial cells in a tubular in vitro system capable of replicating the coronary artery biomechanical environment. In particular, the cellular and molecular changes pertaining to inflammation, proliferation, and death were assessed after stent deployment. Real-time quantitative PCR demonstrated increased expression of genes encoding for E-Selectin, ICAM-1, and VCAM-1; markers associated with an inflammatory response in vivo. Further, an increase in the pro-apoptotic protein Bax was paralleled with a decrease in the anti-apoptotic protein Bcl-2; however, apoptotic morphology was not observed. Interestingly, transcription of c-fos increased, whereas Ki67 levels fell over the same period. One hypothesis is that these results are in response to the altered local hemodynamic environment induced by stent deployment. Most significantly, this study highlights the potential of a biomimetic hemodynamic bioreactor combined with a gene expression analysis to evaluate, with greater specificity, the performance and interaction of stents with the endothelial layer in a controlled environment.
DOI 10.1007/s10439-009-9701-6
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