Peer-Reviewed Journal Details
Mandatory Fields
Glynn, B,Lacey, K,O'Grady, J,Barry, T,Smith, TJ,Maher, M
2008
September
Journal Of Rapid Methods And Automation In Microbiology
Reusable surface plasmon resonance assay for the specific detection of Streptococcus pneumoniae tmRNA
Published
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Optional Fields
SEQUENCE-BASED AMPLIFICATION HYBRIDIZATION ADSORPTION LISTERIA-MONOCYTOGENES IMAGING MEASUREMENTS BIOSENSOR DNA SAMPLES PCR MICROARRAYS ANTIBODY
16
210
221
A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA before being subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10(12) cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.
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