Peer-Reviewed Journal Details
Mandatory Fields
Gundy, S,Manning, G,O'Connell, E,Ella, V,Harwoko, MS,Rochev, Y,Smith, T,Barron, V
2008
November
Acta Biomaterialia
Human coronary artery smooth muscle cell response to a novel PLA textile/fibrin gel composite scaffold
Published
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Optional Fields
Fibrin Polylactic acid Smooth muscle cell Composite scaffold Warp-knit BLOOD-VESSELS FIBRIN GEL TISSUE INFLAMMATION EXPRESSION GENE SPECIFICITY DISEASE MARKERS FAMILY
4
1734
1744
Previous studies have demonstrated the potential of fibrin as a cell carrier for cardiovascular tissue engineering applications. Unfortunately, fibrin exhibits poor mechanical properties. One method of addressing this issue is to incorporate a textile in fibrin to provide structural support. However, it is first necessary to develop a deeper understanding of the effect of the textile on cell response. In this study, the cytotoxicity of a polylactic acid (PLA) warp-knit textile was assessed with human coronary artery smooth muscle cells (HCASMC). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was employed to examine the gene expression of HCASMC embedded in fibrin with and without the textile. Five genes were examined over a 3-week period: smooth muscle a-actin (SM alpha A), myosin heavy chain 11smooth muscle (SM1/SM2), calponin, myosin heavy chain 10 non-muscle (SMemb) and collagen. Additionally, a microarray analysis was performed to examine a wider range of genes. The knitting process did not adversely affect the cell response; there was no dramatic change in cell number or metabolic rate compared to the negative control. After 3 weeks, there was no significant difference in gene expression, except for a slight decrease of 10% in SMemb in the fibrin with textile. After 3 weeks, there were no obvious cytotoxic effects observed as a result of the knitting process and the gene expression profile did not appear to be altered in the presence of the mesh in the fibrin gel. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
DOI 10.1016/j.actbio.2008.05.025
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