Peer-Reviewed Journal Details
Mandatory Fields
Crowley, PB,Matias, PM,Khan, AR,Roessle, M,Svergun, DI
2009
September
Chemistry-A European Journal
Metal-Mediated Self-Assembly of a beta-Sandwich Protein
Published
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Optional Fields
crystal growth macromolecular crowding methionine protein-protein interactions X-ray scattering SMALL-ANGLE SCATTERING CYANOBACTERIUM PHORMIDIUM-LAMINOSUM X-RAY BIOLOGICAL MACROMOLECULES CRYSTAL-STRUCTURE ESCHERICHIA-COLI CYTOCHROME-F PLASTOCYANIN COORDINATION INTERFACES
15
12672
12680
The beta-sandwich cupredoxin Plastocyanin (Pc) was found to self-assemble in the presence of Zn2+, a known mediator of protein-protein interfaces. Diffraction-quality crystals of Pc grew from solutions containing zinc acetate as the sole precipitant. Di- and trinuclear zinc sites contribute to the crystal contacts in this structure. A different crystal form, also involving numerous zinc bridging ions, was obtained in the presence of poly(ethylene glycol) 8000. Comparison of the two crystal forms reveals the effect of macromolecular crowding on self-assembly. Solution-state structural characterisation of the Zn2+-mediated Pc oligomers was performed by using a combination of chemical shift perturbation mapping and small-angle X-ray scattering. The data indicate the formation of dimers in solution. The implications for metal-mediated assembly and crystallisation are discussed.
DOI 10.1002/chem.200901410
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