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Bauerschmidt, C,Pollok, S,Kremmer, E,Nasheuer, HP,Grosse, F
2007
June
Genes To Cells
Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase
Published
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Optional Fields
XENOPUS EGG EXTRACTS MINICHROMOSOME MAINTENANCE PROTEINS ORIGIN RECOGNITION COMPLEX REPLICATION FORKS CHROMOSOMAL DNA SACCHAROMYCES-CEREVISIAE CHROMATIN ASSOCIATION BIND CHROMATIN DISTINCT ROLES FISSION YEAST
12
745
758
Cdc45 is an essential cellular protein that functions in both the initiation and elongation of DNA replication. Here, we analyzed the localization of human Cdc45 and its interactions with other proteins during the cell cycle. Human Cdc45 showed a diffuse distribution in G1 phase, a spot-like pattern in S and G2, and again a diffuse distribution in M phase of the cell cycle. The co-localization of Cdc45 with active replication sites during S phase suggested that the human Cdc45 protein was part of the elongation complex. This view was corroborated by findings that Cdc45 interacted with the elongating DNA polymerases delta and epsilon, with Psf2, which is a component of the GINS complex as well as with Mcm5 and 7, subunits of the putative replicative DNA helicase complex. Hence, Cdc45 may play an important role in elongation of DNA replication by bridging the processive DNA polymerases delta and epsilon with the replicative helicase in the elongating machinery.
DOI 10.1111/j.1365-2443.2007.01090.x
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