Peer-Reviewed Journal Details
Mandatory Fields
Helling, AL,Tsekoura, EK,Biggs, M,Bayon, Y,Pandit, A,Zeugolis, DI
2017
September
ACS Biomaterials Science & Engineering
In Vitro Enzymatic Degradation of Tissue Grafts and Collagen Biomaterials by Matrix Metalloproteinases: Improving the Collagenase Assay
Published
WOS: 11 ()
Optional Fields
tissue grafts collagen biomaterials collagen cross-linking in vitro enzymatic degradation matrix metalloproteinases collagenase assay SKIN FIBROBLAST COLLAGENASE DERMAL SHEEP COLLAGEN ABDOMINAL-WALL RECONSTRUCTION SOURCE ANIMAL AGE CROSS-LINKING EXTRACELLULAR-MATRIX NEUTROPHIL COLLAGENASE INTERSTITIAL COLLAGENASE DUPUYTRENS-DISEASE HYPERTROPHIC CHONDROCYTES
3
1922
1932
Matrix metalloproteinase-1 and -8 are active during the wound healing and remodelling processes, degrading native extracellular matrix and implantable devices. However, traditional in vitro assays utilize primarily matrix metalloproteinase-1 to mimic the in vivo degradation micro environment. Herein, we assessed the influence of various concentrations of matrix metalloproteinase- 1 and 8 (50, 100, and 200 U/mL) as a function of pH (5.5 and 7.4) and time (3, 6, 9, 12, and 24 h) on the degradation profile of three tissue grafts (chemically cross-linked Permacol, nonchemically cross linked Permacol and nonchemically cross-linked Strattice) and a collagen biomaterial (nonchemically cross-linked collagen sponge). Chemically cross-linked and nonchemically cross-linked Permacol samples exhibited the highest resistance to enzymatic degradation, while nonchemically cross-linked collagen sponges exhibited the least resistance to enzymatic degradation. Qualitative and quantitative degradation analysis of all samples revealed a similar degradation profile over time, independently of the matrix metalloproteinase used and its respective concentration and pH. These data indicate that matrix metalloproteinase-1 and matrix metalloproteinase-8 exhibit similar degradation profile in vitro, suggesting that matrix metalloproteinase-8 should be used for collagenase assay.
10.1021/acsbiomaterials.5b00563
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