Peer-Reviewed Journal Details
Mandatory Fields
Owens, P,Phillipson, N,Perumal, J,O'Connor, GM,Olivo, M
2015
December
Biosensors-Basel
Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates
Published
()
Optional Fields
Raman spectroscopy protein sensing surface-enhanced Raman scattering biomarker
5
664
677
In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.
10.3390/bios5040664
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